![]() ![]() elegans to investigate the effect of bacteria on host proteostasis and disease pathogenesis 9, 10. Therefore, simpler organisms, such as Caenorhabditis elegans, are often used as a discovery tool 5, 6, 7, 8. However, the complexity of the human, or even murine, microbiome makes it difficult to conduct studies that would reveal the exact influence of microbes on their host. Recent studies suggest that changes in the microbiome influence the onset, progression, and severity of PCDs 2, 3, 4. As such, the development of effective therapies has been hindered by the lack of knowledge regarding the factors and conditions that contribute to disease onset and progression. ![]() While protein misfolding is recognized as the culprit, the etiology of these diseases is not clear. elegans model.Īge-dependent neurodegenerative protein conformational diseases (PCDs) such as Alzheimer's, Parkinson's, and Huntington's diseases, or amyotrophic lateral sclerosis, are characterized by protein misfolding that leads to aggregation, cell death, and tissue degeneration 1. It is anticipated that these methods can drastically simplify the screening process of large bacterial, genomic, or drug libraries using the C. Though the concept of automation is not entirely unique, the need to standardize such procedures for reproducibility, elimination of bias from manual counting, and increase throughput is high. elegans-based image processing pipeline using CellProfiler, an image analysis software, this method has been optimized to separate and identify individual worms and enumerate their respective aggregates. elegans that express intestine-specific polyQ. Herein, a protocol consisting of worm culturing, image acquisition, and data processing was standardized to support high-throughput aggregate quantification using C. Furthermore, manual foci quantification can introduce bias, as aggregate identification can be highly subjective. Manual aggregate quantification is time-consuming, limiting experimental throughput. Such reporters are often employed as proxies to monitor changes in proteostasis across tissues. ![]() Nematodes that express fluorescently tagged tissue-specific polyglutamine (polyQ) tracts exhibit age- and polyQ length-dependent aggregation characterized by fluorescent foci. elegans and human genomes makes this model an invaluable discovery tool. Additionally, high conservation between the C. Use of the Caenorhabditis elegans nematode model to study PCDs has been justified by its relative ease of maintenance, low cost, and rapid generation time, which allow for high-throughput applications. Though murine models have proven invaluable, they are expensive and are associated with laborious, low-throughput methods. This increased attention has called for the diversification and improvement of animal models capable of reproducing disease phenotypes observed in humans with PCDs. A rise in the prevalence of neurodegenerative protein conformational diseases (PCDs) has fostered a great interest in this subject over the years. ![]()
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